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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 67071
شماره مدرک : ‭پ۵۳۵۴۴‬
شماره راهنما : ‭ت۲۹۳۷‬
سر شناسه : وثوقی، هما
عنوان اصلي : بررسی مقایسه ای میزان بیان ژنهای‭TLR-2‬ و‭TLR-4‬ خون در دو گروه بیماران باسپسیس باکتریال با کشت خون منفی و افراد سالم
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /هما وثوقی
نام ساير پديدآوران : ؛استاد راهنما: حمیدرضا نادری، مریم راستین
نام ساير پديدآوران : ؛استاد مشاور: مهدی جباری
عنوان ديگر : عنوان به انگلیسی‭Evaluation whole blood TLR-2 and TLR-4 gene expression in bacterial sepsis with negative blood culture and normal population :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۲‬، دانشکده پزشکی
صفحه شمار : ‮‭[۱۰۳]‬ ص.‬: مصور
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭shaped) in comparison to healthy control there was significant difference with cutoff ratio of 46.19 and sensitivity and specificity of 87 and 78 , respectively . So, evaluation of TLR4 gene expression may be useful to consider anti staphylococcus antibiotic therapy for patients with culture negative bacterial sepsis. -shaped) but negative blood cultures in comparison to healthy control was significantly lower (p value:0.004) meanwhile, regarding TLR2 there was no difference(p value:0.0928). 4) For other comparisons there were not any significant difference. Conclusion: TLR4 gene expression in patients who had negative blood culture but gram positive cocci smear (not enterococcus and lancet-Timely diagnosis of bacterial sepsis is of great concern. However, regarding the great amount of negative blood cultures this is not an easy goal. Thus, it seems that detection of certain factors such as TLR molecules could be helpful for diagnosis of sepsis and guiding appropriate antibiotics. The aim of this study is comparison of mononuclear blood TLR2 and TLR4 gene expression in culture negative bacterial septic patients with healthy people. Method: This case control study was performed on 30 culture negative bacterial septic patients and 30 healthy people. We also evaluated 5 culture positive patients alternatively. 6 cc blood was punctured and mononuclear cells were isolated and the expression levels of of TLR2 and TLR4 gene was evaluated by Real Time RT.PCR (Taq Man method). The data were analyzed by SPSS soft ware and cutoff ratios were calculated by R soft ware. Result: 1) The expression level of TLR2 and TLR4 genes expression in culture negative and healthy control had not significant difference (p value TLR2 :0.52) and (p value TLR4 :0.099). 2) In comparison, The expression level of TLR4 between total patients (culture positive and negative) and healthy control was significantly differente (p value :0.028). 3)The TLR4 gene expression in patients who had gram positive cocci sample smear (not enterococcus and lancet‬
 
 
 
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