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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 67068
شماره مدرک : ‭پ۵۳۵۴۲‬
شماره راهنما : ‭آ۳۸۲‬
سر شناسه : مرادی، فهیمه
عنوان اصلي : شناسایی پروتئین های برهم کنش دهنده با‭SSAT-1‬ و‭SSAT-X‬ در شرایط هیپوکسی و نورموکسی در رده سلولی‭A172‬
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /فهیمه مرادی
نام ساير پديدآوران : ؛استاد راهنما: مجید مجرد، محمدرضا عباس زادگان
نام ساير پديدآوران : ؛استاد مشاور: محمد حسن زاده نظرآبادی، حسین زرین فر
عنوان ديگر : عنوان به انگلیسی‭Identification of proteins interacting with SSAT-1 and SSAT-X in A172 cell line cultured in normoxic and hypoxic condition :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۲‬، دانشکده پزشکی
صفحه شمار : ‮‭[۱۱۳]‬ ص.‬:مصور
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭X -1, SSAT-1 has possible roles in motility and migration, invasion, cell cycle progression, cell proliferation and differentiation. We hope to use these data to design new therapeutically approach in cancer treatment. Keywords: Glioblastoma, Hypoxia, SSAT-1 is more than a catabolism enzyme in polyamine pathway. SSAT-1 can interact with: foxG1, CyclinD3, Dido1, PTPRU, COG3, RCAN3, CDS2, SLC34A2, and ARF6. Conclusion: SSAT-SSAT fusion protein did not solely localize in cytoplasm as expected but interestingly major portion of SSAT protein was aggregated in nucleus of neoplastic cells.Y2H results showed that SSAT-X plasmids as baits and cDNA libraries as prey. Plasmids encoding bait interacting preys were rescued and their identity was determined using sequencing followed by bioinformatic approaches. Results: Localization results demonstrated that YFP-SSAT-1 or pGBKT7-SSAT-X, Yeast Two Hybrid techniques was applied. pGADT7 based cDNA library was made from A172 cell line cultured in hypoxic or normoxia conditions. Mating was done between yeast strains containing either recombinant pGBKT7-1and SSAT-1 localization in cancer cells using yellow fluorescent protein (YFP) localization tag. In order to identify the proteins interacting with SSAT-X. Method: We first investigated the SSAT-1 or SSAT-X .In this study we try to determine the possible interacting proteins with SSAT-1 or SSAT-1 splice variant and its overexpression increases cell survival under poor condition. Exact mechanisms underlying these effects are not yet fully characterized. One of the most critical steps in this process is identification of biological partners of this protein. Identification of these partners may give us some clues about biological mechanisms involving SSAT-X is another SSAT-1 overexpression in some cancers is associated with a good prognosis and in some with poor prognosis. SSAT-1 overexpression in response to Hypoxia has controversial consequences in different tissue carcinogenesis process. SSAT-Background: Despite of promising advances in therapeutic approaches for Glioblastoma multiforme treatment, this malignancy is still among the most mortal human cancers. In the recent years, temporal microenvironment especially Hypoxia has attracted the attention of many scientists In order to find an effective therapeutic approach against cancer. Hypoxia is thought to be the mechanism by which an aggressive tumor phenotype is developed through increased invasion, loss of apoptosis, chemoresistance, resistance to antiangiogenic therapy, and radiation resistance. Our recent studies indicate SSAT‬
 
 
 
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