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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 66975
شماره مدرک : ‭پ۵۳۴۷۷‬
شماره راهنما : ‭ت۲۶۳۹‬
سر شناسه : برومند نوقابی، سمانه
عنوان اصلي : بررسی تغییر در میزان بیان و توالی ژن درماتوپونتین‭(Dermatopontin)‬ در سرطان کولورکتال
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /سمانه برومند نوقابی
نام ساير پديدآوران : ؛استاد راهنما: ناصر طیبی میبدی، آریانه صدر نبوی
نام ساير پديدآوران : ؛استاد مشاور: محمد رضا عباس زادگان، حبیب الله اسماعیلی
عنوان ديگر : عنوان به انگلیسی‭Evaluation of changes in expression and DNA sequence of dermatopontin gene in colorectal cancer :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۱‬، دانشکده پزشکی
صفحه شمار : ‮‭[۹۹]‬ ص.‬: جدول، نمودار
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭signaling pathway in colon carcinogenesis.-receptor component of TGF- in colorectal cancer, DPT may serve as a pre-s activity (a tumor suppressor which its pathway undergoes changes in about 85 of colon cancers and acts as a tumor promoter in late stages, too), suggesting a tumor suppressor function for DPT. This study was aimed to investigate the changes in DPT gene expression and sequence in colorectal cancer providing better understanding of its carcinogenesis to be used in development of new therapies.Method: 38 newly diagnosed cases of colorectal cancer who were going to undergo colectomy, were enrolled the study. Fresh colonic tumoral and normal specimens were obtained. DPT mRNA expression was analyzed by real time PCR. In cases of DPT under expression, tumoral DNA was extracted and after doing PCR, the genes exons were sequenced by Sanger method using capillary electrophoresis. Results: DPT expression was decreased in 14 cases (36.8 ) of colorectal cancers, while 12 cases (31.6 ) showed over expression and the others had no changes in DPT expression. DPT expression pattern had no significant difference in various grades and stages of tumors. 45.5 of stage III tumors over expressed DPT, whereas only 26.9 of low stage tumors showed over expression (p=0.33). In addition, the frequencies of DPT over expression was higher in tumors having lymph node involvement (47.7 vs. 28 , p=0.59). We observed two cases which had mutations that maybe responsible for the decreased expression of DPT.Conclusion: regarding the similarities between changing patterns of DPT and TGF-s activity and induction of cell quiescence. These roles, especially increase of TGF-Background: Dermatopontin (DPT) is an extracellular matrix protein which plays roles in collagen fibrillogenesis, cell adhesion, increasing TGF‬
 
 
 
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