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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 66887
شماره مدرک : ‭پ۵۳۴۰۳‬
شماره راهنما : ‭آ۳۹۸‬
سر شناسه : مسیب زاده روشن، هانی
عنوان اصلي : کلونینگ و بیان دومن های نوترکیب‭WW3/WW2‬ از مولکول‭SMURF2‬ به عنوان دومن های موثر در پیامدهی‭TGF-B‬
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /هانی مسیب زاده روشن
نام ساير پديدآوران : ؛استاد راهنما: مجتبی سنکیان
عنوان ديگر : عنوان به انگلیسی‭Cloning and expression of ww2\ww3 recombinant domains as effective domains on TGF-B signalling :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۱‬، دانشکده پزشکی
صفحه شمار : ‮‭[۱۰۷]‬ ص.‬: مصور
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭signal transduction can further be investigated. -PAGE . Result: The peptide was successfully expressed in codon plus bacteria and purified by nickel affinity chromatography. The sequencing results of the inserted vector demonstrated that the gene has been inserted in frame into the vector Conclusion: Ww2\ww3 peptide expressed in codon plus prokaryotic cells and separated using Ni affinity chromatography has a molecular weight of 37KD and is considered a cationic peptide in neutral PH due to the presence of TAT sequence . Its effect on TGF-21 codon plus for expression. After expression, the bacteria were lysed and the solution dialyzed. Finally, the peptide under investigation was purified by Ni affinity chromatography and analyzed by SDS-). Recombinant vector was transformed into BL+21b(-beta signaling in living cells, it must enter into cells and for this purpose, tat peptide provides the means) and then the amplified region was inserted into expression vector pET-end of forward primer (Because the final aim of this research project is to explore the impact of ww2\ww3 on TGF-beta signaling cascade. A tryptophan rich sequence by the name of WW2\WW3 within the molecule is accountable for the binding of the molecule to Smad7. This final complex is the active form which can practically play its part in the inhibition. As depicted, it is clear that WW2\WW3 sequence is an essential component to take part in the negative regulation of the signaling, since, it provides a bridge for the assembly of the two molecules into one single active complex. Method: The total RNA was extracted from human PBMC and was reverse transcribed into cDNA. The sequence of interest encoding the peptide was amplified using PCR primers containing TAT sequence at the 5-ubiquitin ligase family of proteins. Its role is in inhibition of TGF-Background: Smurf2 is a member of the E3‬
 
 
 
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