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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 66862
شماره مدرک : ‭پ۵۳۳۷۹‬
شماره راهنما : ‭ع۶۷۸۷‬
سر شناسه : سعیدی، رامین
عنوان اصلي : تعیین فراوانی جهش ارثی جدید در ژن‭BRCA-2‬ در اشکال خانوادگی و اسپورادیک سرطان سلول های سنگفرشی مری
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /رامین سعیدی
نام ساير پديدآوران : ؛استاد راهنما: محمد رضا عباس زادگان
نام ساير پديدآوران : ؛استاد مشاور: مهران غلامین
عنوان ديگر : عنوان به انگلیسی‭Analysis of a novel hereditary mutation in BRCA2 gene in the FAmilial and sporadic cluster of esophageal squamus cell carcinoma :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۱‬، دانشکده پزشکی
صفحه شمار : ‮‭[۸۸]‬ ص.‬: مصور
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭PCR technique cDNA made from patient RNA, Investigation proves that cDNA was smaller than that of the control sample was. Then the target RNA sequences that would indicate a deletion of exon 5. Conclusions: As stated Mutant 426_2A> G was not reported in any of the control samples, and sporadic that only in one family samples and its members is found to be heterozygous. Due to the location of the mutation in exon, The patients were studied RNA sequencing and protein readings that Confirm the deletion of exon 5 and a protein which has 30 amino acid. Key word : esophagus cancer,BRCA2 gen, mutation -2A> G) on 120 patients with esophageal cancer without a family history And 15 patients with a family history and 60 control subjects were studied. In which the samples mutation studied by PCR Technique and RFLP with restriction enzymes acting AVAII. Then, using samples and RT_PCR techniques for monitoring protein resulting from this mutation , cDNA made from patient RNA and DNA sequences were evaluated. Results: This mutation in 120 Sporadic samples and 60 control samples were not reported however in 15 patients there was a family history of esophageal cancer only one case was reported. In this patient, eight of whom are also examining other family members had the mutation. Then by using RT-Introduction: Esophageal cancer is the eighth most common cancer in the world that is the sixth leading cause of death from cancer in worldwide. Areas with high incidence of esophageal cancer are in Central Asia , France, Italy, Brazil, Eastern and Southern Africa. Given the prevalence of this cancer, early detection is very important. A genetic analysis of early diagnostic methods that in this study, Reviews the BRCA2 gene as gene involved in familial esophageal cancer. Materials and Methods: In this study the mutation (426‬
 
 
 
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