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رکورد قبلیرکورد بعدی
نوع مدرک : TF
زبان مدرک : فارسی
شماره رکورد : 66846
شماره مدرک : ‭پ۵۳۳۷۰‬
شماره راهنما : ‭آ۳۳۴‬
سر شناسه : شریعتی کوهبنانی، مهدی
عنوان اصلي : بررسی تاثیر تجویز نیکوتین در دوره بارداری بر بیان لامینین و فیبرونکتین پارانشیم ریه نوزادان موش
نام عام مواد : [پایان‌نامه]
نام نخستين پديدآور : /مهدی شریعتی کوهبنانی
نام ساير پديدآوران : ؛استاد راهنما: محمد رضا نیکروش، مهدی جلالی
نام ساير پديدآوران : ؛استاد مشاور: علیرضا فاضل، علیرضا ابراهیم زاده بیدسکان، مجتبی سنکیان
عنوان ديگر : عنوان به انگلیسی‭Study of Laminin and Fibronectin expression following maternal nicotin exposure in lung parenchyma of the newborns mouse :‬
وضعيت نشر : دانشگاه علوم پزشکی مشهد، ‭۱۳۹۱‬، دانشکده پزشکی
صفحه شمار : ‮‭[۱۶۳]‬ ص.‬: مصور
يادداشت : چکیده فارسی، چکیده انگلیسی
يادداشت : چاپی
خلاصه يا چکيده : ‭values <0.05 were considered statistically significant. Results: Our finding reveled that lung and body weight was significantly decreased near offsprings whose mothers were exposed to nicotine on days 1 and 14 compared to control groups (P<0.01). Real time investigation showed that relative mRNA expression of fibronectin in experimental group 1 and 2 was 1.02 and 1.72 respectively while relative mRNA expression of laminin was 0.63 and 1.36 respectively. Immunohistochemistry studies also indicated that intensity of immunoreactivity was not similar in the different parts of the lungs including alveoli, bronchiole and small vessels in studied groups relative to anti fibronectin and anti laminin in experimental animals. Intensity of immunoreactivity related to anti fibronectin in experimental group 1 in comparison with control group showed increase in fibronectin distribution in extracellular matrix of the alveoli and small vessels but decrease in the bronchiole (P<0.05). In experimental group 2, fibronectin distribution among the alveoli and small vessels increased from average to high compared with control group but no significant change was observed in bronchioles. Immunoreactivity intensity related to laminin antibody in distribution group1 and 2 showed that laminin distribution significantly increased (P<0.05) in alveoli, bronchioles and small vessels of experimental group 1 compared with control. Laminin distribution increased significantly (P<0.05) in alveoli and bronchioles of experimental group1 whereas in small vessel no significant changes was observed. Conclusion: Results of this study show that maternal nicotine administration may induce abnormal fibronectin and laminin expression in newborn lung. These changes were observed in alveoli and bronchioles during pregnancy and lactation periods. Most likely observed changes could have effect on lung development which might conclude in its abnormal function. Therefore although smoking prevention is strongly recommended during pregnancy, attention to breastfeeding period is also necessary. Keywords: nicotine, fibronectin, laminin, lung, mice. -parametric statistical test, P-Whitney non-test and Mann-Background: Maternal exposure to smoke has been associated with pregnancy complications in both human and experimental animals which are included spontaneous abortion, intrauterine retardation (IUGR), fetal and neonatal death and premature delivery. It has been shown that nicotine a major constituent of tobacco easily crosses the placenta barriers and could concentrate in different developing tissues and might interfere with many developmental phenomena such as respiratory system maturation. In this study, expression and distribution of fibronectin and laminin, two key elements of lung development, after maternal exposure to nicotine, among newborn mice, has been evaluated. Material and methods: Pregnant Balb/C mice were randomly divided into two experimental and two control groups. The experimental group 1: from day 7 of gestation until birthday and experimental group 2 during the same gestational days up to post natal day 14 received 3 mg/kg intraperitoneal nicotine. Control groups 1 and 2 were also received the same volume of normal saline during the same periods. All newborns were anesthetized with chloroform, lung weight was measured and tissue preparation was processed for immunohistochemical and Real time PCR techniques. Results were analyzed using student t‬
 
 
 
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